P-242: Sex Determination in ICSI Oocytes with Two Polar Bodies Using PGD and FISH, A Case Report

Background: Morphology and presence of Polar body in the oocyte are denoting oocyte quality and is used as a criterion for prognosis of embryo quality. However, little is known about ICSI result of oocytes with two polar bodies. The present study reports the result of ICSI in a case having several oocytes with two polar bodies. Materials and Methods: A 34 years old patient (K.H.) with history of familial marriage admitted to Mother's Clinic in Tabriz-Iran for preimplantation sex determination. A total of 12 oocytes was collected after superovulation protocol. From the collected oocytes 7 were at MII stage and 5 had 2 pollar bodies. All oocytes including those with 2 polar bodies were underwent ICSI procedure, but were kept separate from MII oocytes. Biopsy procedure was performed on day 3 and FISH method, based on single cell model, was used for sex determination. Results: The results revealed that all oocytes, in both groups, were fertilized and developed to embryos. The embryos had almost similar morphology in both groups and appeared normal. However, the result of FISH signals for biopsied blastomere showed that in MII group, all embryos were XX/XY but one was XO. However, in 2 polar body group one embryo was XO and the other four were NS. Conclusion: It is concluded that the embryos obtained from oocytes with two polar bodies should not be transferred.